This week, I designed an in vitro conditioned media experiment to test the effects of parathyroid gland (PTG)-derived CD34+ cells on stressed human islets. To stress these cells, I cultured them for 24 hours in either replete media (control), 1% diluted media, 1% oxygen (to induce hypoxia), or both stress factors. I added SCIPCs, PTG cells, CD34- cells, or CD34+ cells to one of each category, resulting in total of 16 wells. This is the image of the well plates in the incubator for overnight culture. The next day, I looked at the conditions of the cells using a microscope and I could visibly see that islets in wells containing CD34+ cells were doing much better than all the other wells. Then, I stained the cells using propidium iodide (PI), which binds to the DNA. However, it cannot cross the membrane of live cells and thus only stains dead cells. With PI staining, I could quantify cell viability of each well using flow cytometry. As I have seen wit...