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Showing posts with the label Week 8-9

Michelle Lu, my last week

With only one week left in the lab, Anna and I are rushing to collect as much data as we can before I leave. After spending basically all of July staining and imaging, we can finally devote some time to quantifying those images. Fortunately, we can pull a lot of data from the images we've collected through a program called Imaris. Unfortunately, some of that data must be collected by hand counting. For example, in order to determine the total number of sensory terminals and how many GABApres are on each sensory terminal, we must go through the Z-stack on the computer and quite literally count how many blue dots there are and how many yellow dots are on each blue dot. Sounds fun, doesn't it? The best part is that each image takes an average of 3 hours to quantify and we have 18 images to do!! So much fun!! But in all seriousness, the data analysis portion of this project is actually quite interesting, for comparing one simple thing, like the difference in size of the sensory ter...