Srinidhi Baile, Doing Things On My Own

During my third week in the lab, I got the chance to do some work instead of just learning techniques. On Monday, I got to make the media for the HepG2 cells myself. This is media contains all the things the cells need in order to grow and proliferate for a few days. On Tuesday, both the grad students I follow were out sick. However, this was a problem because Danyelle, one of the students, needed to be in the lab to finish her western blot. This left Dara, a masters student, and I to finish it for her. Although it wasn’t a lot of work, Danyelle believed we were capable enough to finish her work for her. Later in the week, I started to do more and more things by myself. I got to run my own western blot (just with old samples), and I was excited to see that it worked (mostly). I also got to do a transfection with two plasmids, which also worked well. I’ve learned so much in my 3 weeks here, and I can’t wait for 3 more.

Western Blot

GFP transfection after 3 days

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Peddie EXP Summer 2018

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