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Srinidhi Baile, Nearing the End

The past few weeks in the lab were really fun. About 2 weeks ago, the lab welcomed 3 more summer students, which was nice because I wasn’t the newest one around anymore. It was cool to both be learning from my grad students and to be teaching the new student things, even if they were small. I started to truly enjoy coming to work everyday, not only because of the science, but because my lab became a little community and I was a part of it. Lab members weren’t just lab members— they were friends now too. I am really going to miss being here.

One issue I faced was not knowing when I would start my project. As time went on, my project kept changing so that it would be something I could complete in my remaining time. I am currently working on determining if there is a difference in the localization of AKT (a protein kinase that has many functions in the cell) if the presence of HBV. To do this, I will be transfecting cells under different conditions, and then counting cells to see where AKT localizes. This can be done because AKT is fused with GFP, a green fluorescent protein. We can use a fluorescent microscope to detect the green, and therefore the AKT.

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