This week has been nice in that it has been a mix between
reading literature and working with Mat Lab.
After presenting another PowerPoint and fixing loose ends, I finalized
the procedure and task for my project.
However, I now have to code the task that I will be performing on the
participants. With no prior programming
experience and very little help in my lab, I have turned to YouTube videos focusing
on Python. However, I should be finished
coding sometime next week, just in time to start testing and analyzing
data. This summer is going by quick!
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
Comments
Post a Comment