In terms of activities outside of the lab living in Berkeley with Alan has been fun, our apartment building has a Trader Joe's on the first floor, which means groceries are not an issue. We also have a washer and dryer in our apartment, which is also another key convenience aspect. Over the weekend we went to San Francisco and explored the city.
As mentioned in my previous blog, I have been awaiting ligament, menisci, and cartilage tissue from a canine or human knee joint for (interleukin) IL-1B tissue culture. IL-1B is an inflammatory cytokine that has been proven to increase rates of tissue degeneration and osteoarthritis development in the Thompson Lab. Dr. Stoker wants me to experiment with different types of knee tissues in a co-culture with varying levels of this cytokine to determine its effects on the entire knee joint. This co-culture uses an insert permeable to the media to separate the two tissue samples from physical contact, while allowing them to share the same media. This creates an extremely accurate model for knee tissues in their native environment due to their exposure to the same synovial fluid in the joint. This model would then be treated with the IL-1B and cultured for 21 days. During these 21 days, the media would be collected every three days for biomarker evaluation at the end of the stu...
Nice explanation of your work so far! Sounds like you are catching on to the coding quickly!
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