After being out of the country for
nearly three months I am sitting In the Sydney Airport ready to board my flight
back to the US. I still can not believe what an amazing experience I had and
how welcoming my lab was. I was able to help out with amazing projects that I
would never have had the change to be a part of without EXP. I learned what
types marine biology I do and don’t enjoy, and I got to see what it was like to
work as a researcher in a laboratory setting.
I am so grateful for this opportunity and I can’t wait to share my
experience with the Peddie community.
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
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