It has been exactly a month after the experience at UCLA. Looking back, even though, at first, I didn't really know if I was going to be a big help to the lab. However, looking back, I have accomplished so much more than I expected. I finished the project that my PI provided me and I went beyond that and I helped other projects too. As a future chemistry major in college, I believe that this experience was one of a kind and it was a big leap forward in my life. Thank you for this experience.
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
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