First day at the lab wasn’t the best. I somehow walked into 5 different buildings between 8 and 8:30 when I had to be there by 8:30. I asked about 5 different people where Dr. Caram’s office is and I finally found his office around 8:40, which was 10 minutes late. I explained to him how I had a hard time finding it and he said it was fine. Dr. Caram was really nice and enthusiastic about everything. After giving me a basic tour around the lab, it was around 9 and he said he needs to go teach a class. So he basically left me in the lab space with nothing and I was just alone. Other people started to show up around 9:30. I tried to talk to a lot of them. Our group consists of 8 people. Tim, the graduate student that I have to shadow or the student that will give me the most help with, helped me download Labview, the coding program used here, on my computer. Even though I did some tutorials before I came here, I still didn’t know how to do 99.9% of the things on here. My graduate student gave me two simple yet really difficult tasks for me to do. It took me about an hour and a half to do it. I felt really stupid and I just feel like I am such a burden to this group rather than a help. But, when I finished my task, I was really happy and I felt accomplished. After all these simple coding tasks I feel like I learned a lot too. Also, I attended my first lab group meeting which consisted of two presentations from the students. To be honest, I didn’t understand anything, but it was a valuable experience because I got to know most of the people in the group.
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
Glad you finally found the right office! I am sure your labview skills will quickly improve! (You can always work on that in the evenings if you feel like your lack of ability is hindering your ability to be helpful to the lab!)
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