During Week 5, I worked on connecting the two equipments: the hydra harp and the stages. The hydra harp is the equipment that counts the photons when the sample is excited, and the stages is a moving microscope that examines the sample. Therefore, in my code, I inputted the movement of the stages, which is 3 dimensional, then I allowed it to count photons while it moves to different locations. Dr. Peretz visited me this week, and it was really nice for me to show her what I’ve been doing so far and how the lab environment is. I can’t believe 5 weeks have already passed and I only have one more week.
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
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