Skip to main content

Kylie, First Week

I’m about to wrap up my first week in the lab and I learned that Californians do in fact use the word “gnarly” as much as one would think.



The graduate student's paper!
View from the lab
My lab is located on the UCLA campus right next to Westwood, which is a great spot to grab food or go shopping (basically I’m in my happy place). And everything here in Los Angeles is vegan! Even my PI! I was a little bit worried that I’d be living off Clif Bars for lunch, but the students in my lab are so welcoming and friendly that I have been blowing through my allowance easily by going out to eat almost twice a day, everyday so far. I feel so at home in my lab--the people are amazing, the location is great (and sunny), and the science is incredible. Before starting my internship I was a little concerned that I would be placed in a corner to pipette from 9-5, but I got more than I could ever hope for. The graduate student whom I am shadowing, Preston, explains everything in so much detail and so clearly. On the first day, he already let me handle the membrane after we transferred the protein from our gel electrophoresis. The western blot, a method that Dr. Goldstein wants me to master, is much more complicated than I initially anticipated (which makes it more fun to do, of course). I am already assigned a project that follows up on the results that Preston and I collected yesterday to do on my own. Today, we had our first lab meeting. There are ten lab members total. One of them is a girl named Blake, who has the exact same dates as me. Another, Johnny, is an undergraduate student who has been a big help me--I’ve never met someone so passionate before. I printed out a paper today on the relationship between HoxB13 and an androgen receptor splice variant so I could familiarize myself with the field a little more. When I checked the authors, it ended up being the first paper of the graduate student sitting next to me! I nerded out a little bit.
I love the fact that I can talk about the lab’s research so easily. Thanks to my preparation through EXP and the help of Dr. Peretz during spring term, I’ve been able to follow most of the really complicated experiments and discussions well--I’ve even surprised my grad student. Not only is my understanding of the field a little bit of a leg up in the lab, but it has let me actively engage in conversations with my peers. I don’t think I can get away with talking about science like this back at home.
Another note--after only being here for a week, I’ve learned a lot about people in general. I’ve only heard a few stories so far, but this lab experience has really expanded my world view. Not everyone takes the expected path to get where they want. I hope to get some more life lessons along the way…

Hopefully next time I blog I can share some details about a comparison between primary basal and luminal cells and those cell types in organoid structure in their expression of MEII!

Comments

  1. Sounds like you are making a smooth transition to the lab! I am happy you have lots of food options but you are in a great place for that! Looking forward to reading more about your experiences in lab.

    ReplyDelete

Post a Comment

Popular posts from this blog

Kylie Heering, Week 2 at the Goldstein Lab

We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...

Alan - First Week at UCSF

Hi Everyone! After arriving in San Francisco last Sunday, I spent this past week settling into the downtown Berkeley apartment that I’ll be sharing with Rohit for the next couple of months, as well as learning my way around the Roy lab at UCSF. First day at the lab was really exciting. Here are a couple pictures of the Mission Bay campus, which was completed just a few years ago. Everything is super new and modern, and there’s still construction for other buildings going on around the campus. Most of the people who work at the Mission Bay campus are either professional researchers or doctors/nurses for the nearby hospital. The graduate students take most of their classes at the original Parnassus campus (where Maya is). I work in Byers Hall, which is connected to Genentech Hall and a short walk down the block from the shuttle stop. There are three other volunteers working for the Roy lab this summer – Kimmai, David, and Pujita, who are all undergrad college students...

Wendy Li, Week 1

It is now early July and I have finally started my lab work. I arrived there at about 9 am on the very first day of my lab and found out that there were only two people in the office—Alex, a graduate student in engineering school, and me. “There should be more people in the office, but most of them went to a vacuum workshop today.” Alex told me. My work officially started at 10:30 am when my post doctor Subarna came to the lab. Familiarizing me with all the facilities in lab, Subarna first gave me a lab tour. Meanwhile, he showed me all the basic operations with vacuum chamber, ellipsometer, as well as the spin coater. During the rest of this past week, I was in the process of making my own films. I learned to cut Si wafer into 1*1 cm pieces and clean the surface of these Si wafer with duster and plasma which can effectively clean up all the extra organic particles from the wafer. Further, I prepared 10 percent polystyrene (PS 8000) toluene solution as the material for spin coating. ...