This week's mostly been about statistical analysis, which was great at first until we realized that we didn't have enough rat subjects for our data to be statistically significant, which means...more scoring!!(yay) I'm not complaining, but I really would have loved to see some solid results. No one else was as disappointed, so I guess this pace at the lab is the norm. This means, however, that I probably won't be able to be there when the statistics actually end up being significant enough to establish a conclusion. Other than that, I found a really great Thai restaurant just around the corner of where I've been living, but I'm leaving on Wednesday, so I think I'm just gonna pig out every day until I actually have to leave :(
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
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