I was able to collect a lot more data outside of the office this week which was great because it gave me more in-lab work to do as a result. Nick came back this week and helped me figure out the problem I was having last week and we also had a long conversation where I was able to ask him a bunch of questions about the work I am doing. On another note, after all the primary literature that we were required to read in class for EXP, I never thought I would be in a position that I would readily WANT to read primary lit. But the office has so many piles of literature that people have collected over the years, and every paper I see seems more fascinating than the last. I've been able to read stuff on the topic that I am working on but I have also been able to read about other aspects of the field of child psychology. Dr. Lewis should be back in the office next week and I'm excited to touch base with him!
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
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