Skip to main content

Emma Morin, Swarm Lab weeks 5 and 6

Week 5 and 6

Hi again! These last two weeks have been great...and by great I mean kind of hectic and extremely tiring. On the bright side, I have been very independent in my lab work, and I have had a lot of fun doing it. The camera focusing issue I mentioned last week has all but disappeared, and I am able to begin experiments on my own now. However, there have been a few hiccups. The first one we found was that the 10% agar in the experimental plates was losing water and shriveling during the experiment. This messed with the results and quickly became an issue that we needed to fix quickly. Subash 3-D printed three different punches until we finally found one that could fit inside the plate without breaking the agar and wouldn’t shrink. Additionally, the tubules were truly testing my patience because they wouldn’t grow nicely, and many of them had bumps and junctions that made them unusable. (I must note that we finally got new small scissors on 8/1/18, and they are much better at ridding the tubules of bumps)
Some days I would come all the way to Newark only to find that there was no growth, and I would wait for a few hours to see if there was any progress, and eventually go home having done nothing. The worst day in my lab to date occurred this Monday. That day, I had to attend the NJIT Undergraduate Research Symposium. Having arrived early to campus, I decided to sit down in the lounge. Now, this lounge has some very comfy chairs that happen to be directly under a wooden ledge.

‘Hm’, I thought, ‘Someone could hit their head on that ledge’ My instincts seem to be quite sharp. I strode over to the chair, and I threw my tired body into the seat, immediately whacking my head into the ledge with full force. My coordination and general intellect seem to be less so. Long story short, I started to cry from the pain and hid in the bathroom until it subsided to a dull ache. I had headaches for the rest of the day. When I finally sat on the train, I felt better. At least I would be home in 45 minutes. I showed my ticket to the conductor and he laughed and informed me that I had boarded the wrong train, and would have to get off in New Brunswick and wait for the next train to Hamilton. Sweet. When we finally got to New Brunswick, I waited for another 40 minutes for the next train to Hamilton. My only saving grace, the only silver lining of the day, was that the train home was the cool one with wood panels and leather-esque seats. I, carefully this time, sat on the train, took a few more painkillers, and finally went home.
After a day like that, every other day is a bit brighter in comparison. The rest of this week has consisted of preparation for the NJIT Provost High School Summer Research Symposium, where I will be presenting my research. I'm actually writing this the day of the symposium, so I'm a bit nervous but excited nonetheless. I still have lab work to do though, so once I post this I have to start replating the slime molds. I might as well get that done now, see you soon!

Comments

Popular posts from this blog

Kylie Heering, Week 2 at the Goldstein Lab

We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...

Alan - First Week at UCSF

Hi Everyone! After arriving in San Francisco last Sunday, I spent this past week settling into the downtown Berkeley apartment that I’ll be sharing with Rohit for the next couple of months, as well as learning my way around the Roy lab at UCSF. First day at the lab was really exciting. Here are a couple pictures of the Mission Bay campus, which was completed just a few years ago. Everything is super new and modern, and there’s still construction for other buildings going on around the campus. Most of the people who work at the Mission Bay campus are either professional researchers or doctors/nurses for the nearby hospital. The graduate students take most of their classes at the original Parnassus campus (where Maya is). I work in Byers Hall, which is connected to Genentech Hall and a short walk down the block from the shuttle stop. There are three other volunteers working for the Roy lab this summer – Kimmai, David, and Pujita, who are all undergrad college students...

Jaewon Oh - Week 7 and 8

Finally done here with my experience and I wish I had more time keep researching so that I have something a little more "finalised" to present. But I guess that's what past EXP kids meant when they said that 8 weeks of research is not enough and I'll have to work with what I've got. To solve the problem of not having enough data points, we used the online TCGA database for raw data that would be used to calculate mutation rates. Mutation rates were calculated through an R coding script that Dr. Cannataro had made. Because the mutation rates were tumor specific, we had to change the proportions that were obtained from the IARC database using data from another database called cBioPortal. Basically we had to multiply the number of times a certain variant was seen in the IARC database by the percentage of tumors that have a tp53 mutation, because our mutation rates are calculated across all tumors in specific cancers (confusing, I know). After graphing the mutatio...