This week I am in the field
catching harks at rays at Orpheus Island where there is a research station
owned by James Cook University. Each day we get up early (around 5:30-6am)so
that we can get some work done before low tide at 12pm. We take all out of gear
and equipment and place it on an ocean kayak which we drag along with us as we
search for rays and baby black tips in the mangroves. Once we spot one we place
a drop net around it so that it is trapped in a circle and then we use a hand
set to take it out. We determine and record the species, sex, disc width, catch
time, and GPS coordinates of the catch location. We then tag it and take a
tissue sample, a genetic sample, and if possible a blood sample. One person
then runs back on to the island with the blood sample so that it can be placed
in the centrifuge immediately before the blood can coagulate. The other people
then release the tagged ray and record the release time and what state the ray
or shark is in, for example is it swimming away, it is not moving, or worst
case scenario, is it dead? I’m only on my second day here and so far we have
caught 8 rays and 5 blacktips. Tomorrow we will head to another nearby bay on
the island and hope to find some new animals to tag.
We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
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