This week I am in the field
catching harks at rays at Orpheus Island where there is a research station
owned by James Cook University. Each day we get up early (around 5:30-6am)so
that we can get some work done before low tide at 12pm. We take all out of gear
and equipment and place it on an ocean kayak which we drag along with us as we
search for rays and baby black tips in the mangroves. Once we spot one we place
a drop net around it so that it is trapped in a circle and then we use a hand
set to take it out. We determine and record the species, sex, disc width, catch
time, and GPS coordinates of the catch location. We then tag it and take a
tissue sample, a genetic sample, and if possible a blood sample. One person
then runs back on to the island with the blood sample so that it can be placed
in the centrifuge immediately before the blood can coagulate. The other people
then release the tagged ray and record the release time and what state the ray
or shark is in, for example is it swimming away, it is not moving, or worst
case scenario, is it dead? I’m only on my second day here and so far we have
caught 8 rays and 5 blacktips. Tomorrow we will head to another nearby bay on
the island and hope to find some new animals to tag.
As mentioned in my previous blog, I have been awaiting ligament, menisci, and cartilage tissue from a canine or human knee joint for (interleukin) IL-1B tissue culture. IL-1B is an inflammatory cytokine that has been proven to increase rates of tissue degeneration and osteoarthritis development in the Thompson Lab. Dr. Stoker wants me to experiment with different types of knee tissues in a co-culture with varying levels of this cytokine to determine its effects on the entire knee joint. This co-culture uses an insert permeable to the media to separate the two tissue samples from physical contact, while allowing them to share the same media. This creates an extremely accurate model for knee tissues in their native environment due to their exposure to the same synovial fluid in the joint. This model would then be treated with the IL-1B and cultured for 21 days. During these 21 days, the media would be collected every three days for biomarker evaluation at the end of the stu...
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