Skip to main content

Sarah, Week 7-8

As I was wrapping up my time at the lab, Claire was also wrapping up some of her work to present to her thesis committee.  My seventh week was a continuation of probing western blots with different antibodies after extracting protein from the heart, lung, and brain of each of the mice which had been genotyped before.  This allowed for the comparison of heterozygous, homozygous, and wild type mice.  The results from the antibodies were unexpected, leading to some confusion.  Thus, we had to go back and redo the genotyping in order to confirm the original conclusions from the gel electrophoresis.  The genotyping was correct, but there were a variety of other factors which could have led to the unexpected results.
My 8th and final week was spent doing some lab work, but mostly reading some primary literature in order to prepare for my poster.  I took some notes and discussed what I read with Claire in order to gain confidence in my knowledge of the topic.  My second to last day, my lab got cake for myself and the other summer students.  It was really nice to be able to sit down with everyone before I left.  My last two weeks were bittersweet because on the one hand I was excited to be able to just stay at home, but on the other hand I was sad to be leaving the lab after having learned so much in such a fun environment.  I would love to do some more research in college and beyond, but I will have to wait and see where the future will take me.
Labels:

Comments

Post a Comment

Popular posts from this blog

Kylie Heering, Week 2 at the Goldstein Lab

We started off our week with a congratulatory acai bowl trip to celebrate Preston’s acceptance into a training grant program. Acai bowls in California top Playa Bowls (no question about it). From what I can tell, its a pretty huge honor to be recognized by this grant, but he’s really humble about it. On Monday, Preston and I decided that testing antibodies that have never been tested on prostate epithelial cells before would be a good objective for my first Western blot on my own. We needed to probe for ASCT2, a glutamine transporter, and GLS in order to determine if their corresponding antibodies are functional. Antibodies are crucial for Western blots because they bind to the protein of interest (POI), allowing for us to qualify its expression after imaging. As such, Preston wanted to make sure they worked by probing for ASCT2 and GLS on three different cell lines. Cell lines are commercially purchased human cells that have been immortalized (modified to grow indefinitely) by telome...
Post a Comment
Read more

Alan - First Week at UCSF

Hi Everyone! After arriving in San Francisco last Sunday, I spent this past week settling into the downtown Berkeley apartment that I’ll be sharing with Rohit for the next couple of months, as well as learning my way around the Roy lab at UCSF. First day at the lab was really exciting. Here are a couple pictures of the Mission Bay campus, which was completed just a few years ago. Everything is super new and modern, and there’s still construction for other buildings going on around the campus. Most of the people who work at the Mission Bay campus are either professional researchers or doctors/nurses for the nearby hospital. The graduate students take most of their classes at the original Parnassus campus (where Maya is). I work in Byers Hall, which is connected to Genentech Hall and a short walk down the block from the shuttle stop. There are three other volunteers working for the Roy lab this summer – Kimmai, David, and Pujita, who are all undergrad college students...
1 comment
Read more

Wendy Li, Week 1

It is now early July and I have finally started my lab work. I arrived there at about 9 am on the very first day of my lab and found out that there were only two people in the office—Alex, a graduate student in engineering school, and me. “There should be more people in the office, but most of them went to a vacuum workshop today.” Alex told me. My work officially started at 10:30 am when my post doctor Subarna came to the lab. Familiarizing me with all the facilities in lab, Subarna first gave me a lab tour. Meanwhile, he showed me all the basic operations with vacuum chamber, ellipsometer, as well as the spin coater. During the rest of this past week, I was in the process of making my own films. I learned to cut Si wafer into 1*1 cm pieces and clean the surface of these Si wafer with duster and plasma which can effectively clean up all the extra organic particles from the wafer. Further, I prepared 10 percent polystyrene (PS 8000) toluene solution as the material for spin coating. ...
Post a Comment
Read more